ABSTRACT
Mulungu (Erythrina verna Vell., Fabaceae.) is an arboreal specie native to southeastern Brazil, used for medicinal purposes. This plant is characterized by the presence of alkaloids that have demonstrated anxiolytic activity. Due to this activity, this plant is listed by the Brazilian National Program of Medicinal Plants and Herbal Medicines. However, bibliographic information is lacking regarding this species. This study aims to describe E. verna barks, macro and microscopically, as well as some physicochemical parameters for the quality control of its raw material. In addition, the chromatographic profile of its alkaloid fraction and optimization of extraction methods for crude extract production has also been performed.
Mulungu (Erythrina verna Vell., Fabaceae.) es una especie arborea nativa del sudeste de Brasil, utilizada con fines medicinales. Esta planta se caracteriza por la presencia de alcaloides que han demostrado actividad ansiolítica. Debido a esta actividad, esta planta se encuentra en la lista del Programa Nacional Brasileño de las Plantas Medicinales y Medicinas Herbarias. Sin embargo, la información bibliográfica es escasa con respecto a esta especie. Este estudio tiene como objetivo describir E. Verna corteza, macro y microscópicamente, así como algunos parámetros físico-químicos para el control de la calidad de la materia prima fresca. Además, el perfil cromatográfico de la fracción alcaloidica y la optimización de métodos de extracción para la producción de extracto crudo también fue realizada.
Subject(s)
Alkaloids/chemistry , Erythrina/anatomy & histology , Erythrina/chemistry , Brazil , Chromatography, High Pressure Liquid , Erythrina/ultrastructure , Plants, Medicinal , Quality ControlABSTRACT
Alpinum isoflavone was isolated from the n-hexane extract of stem bark of Erythrina stricta. The structure of the compound was elucidated by extensive spectroscopic studies and comparision with published spectroscopic data.
A partir de un extracto, obtenido con n-hexano de corteza de Erythrina stricta, se aisló la alpinum isoflavona. La estructura del compuesto fue determinada por métodos espectroscópicos y comparación con datos espectroscópicos publicados.
Subject(s)
Erythrina/chemistry , Flavonoids/isolation & purification , Spectrum AnalysisABSTRACT
Erythrina velutina (EV) and Erythrina mulungu (EM), popularly used in Brazil as tranquilizing agents, were studied. The effects of acute and chronic oral treatment with a water:alcohol extract of EV (7:3, plant grounded stem bark; acute = 100, 200, 400 mg/kg; chronic = 50, 100, 200 mg/kg) were evaluated in rats (N = 11-12) submitted to the elevated T-maze (for avoidance and escape measurements) model of anxiety. This model was selected for its presumed capacity to elicit specific subtypes of anxiety disorders recognized in clinical practice: avoidance has been related to generalized anxiety and escape to panic. Additionally, animals were treated with the same doses of EV and EM (water:alcohol 7:3, inflorescence extract) and submitted to the forced swim test for the evaluation of antidepressant activity (N = 7-10). Both treatment regimens with EV impaired elevated T-maze avoidance latencies, without altering escape, in a way similar to the reference drug diazepam (avoidance 1, mean ± SEM, acute study: 131.1 ± 45.5 (control), 9.0 ± 3.3 (diazepam), 12.7 ± 2.9 (200 mg/kg), 28.8 ± 15.3 (400 mg/kg); chronic study: 131.7 ± 46.9 (control), 35.8 ± 29.7 (diazepam), 24.4 ± 10.4 (50 mg/kg), 29.7 ± 11.5 (200 mg/kg)). Neither EV nor EM altered measurements performed in the forced swim test, in contrast to the reference drug imipramine that significantly decreased immobility time after chronic treatment. These results were not due to motor alterations since no significant effects were detected in an open field. These observations suggest that EV exerts anxiolytic-like effects on a specific subset of defensive behaviors which have been associated with generalized anxiety disorder.
Subject(s)
Animals , Male , Rats , Anti-Anxiety Agents/therapeutic use , Anxiety/drug therapy , Behavior, Animal/drug effects , Depression/drug therapy , Erythrina/chemistry , Phytotherapy , Acute Disease , Chronic Disease , Disease Models, Animal , Maze Learning , Plant Extracts/therapeutic use , Rats, Wistar , Reaction Time/drug effectsABSTRACT
De la corteza y flores de Erythrina fusca Loureiro se aislaron los alcaloides isoquinolínicos (+) -epieritratidina y 8-(+)-oxoerisodina, los cuales fueron identificados con base en sus constantes espectroscópicas. Se determinó la actividad antimicrobiana de extractos crudos y fracciones frente a bacterias y hongos. Se evaluó la actividad farmacólogica de extractos crudos y fracciones frente a ratas tipo WISTAR
Subject(s)
Rats , Erythrina/chemistry , Isoquinolines/pharmacologyABSTRACT
A lectin was purified from seeds of Erythrina velutina forma aurantiaca by affinity chromatography on cross-linked guargum. The lectin is a potent agglutinin for human (minimal concentration of protein able to cause visible agglutination of a 2 per cent erythrocyte suspension varying from 1 to 4 mug/ml), rabbit(4 mug/ml) and chicken erythrocytes (8 mug/ml) but presented low activity against cow (250 mug/ml) or sheep (333 mug/ml) blood cells. Hemagglutination of human O+ erythrocytes was inhibited by D-lactose (0.2 mM) > D-galactose(0.8 mM) > D-raffinose (2.1 mM). At pH 7.5, chromatography on a Superose 12 HR 10/30 column showed that the lectin was primarily a dimer (56.0 kDa) composed of two identical subunits (31.6 kDa each). A small amount of a tetrameric form was also apparently present. The lectin is a glycoprotein (7.3 per cent carbohydrate), has a pI of 4.5, contains high levels of acidic (Asp and Glu, 64.2 and 51.6 residues/mol, respectively) and hydroxy amino acids (Ser and Thr, 42.9 and 38.5 residues/mol, respectively) but relatively low amounts of sulfur amino acids (Cys and Met, 1.0 and 5.0 residues/mol, respectively) and has an N-terminal sequence of Val-Glu-Thr-Ile/Leu-Pro-Phe-Ser. Its hemagglutinating activity was abolished by heating at 70 degrees Celsius for 10 min. The activation energy (delta G') required for denaturation measured by loss of hemagglutination activity was 24.87 kcal/mol. In rats, the purified lectin (100 mug) induced neutrophil migration into the peritoneal cavity (3.7 ñ 0.6 x 10(6) neutrophils/ml) or into the air pouch (2.75 ñ 0.25 x 10(6) neutrophils/ml), 8 and 10 times greater than the negative control, respectively.
Subject(s)
Humans , Animals , Rats , Erythrina/chemistry , Lectins/chemistry , Brazil , Lectins/isolation & purification , Rats, Wistar , Seeds/chemistryABSTRACT
La lectina de la semilla de E. costaricensis se purificó a partir del extracto crudo mediante filtración por Sephadex G-100 y cromatografía por DEAE-Sephadex A-50. La electroforesis en gel de acrilamida (PAGE) demostró la presencia de una única banda proteica. El isoelectroenfoque analítico demostró la presencia de cuatro isolectinas. La masa relativa obtenida por filtración en Sephadex fue de 58 KDa y por PAGE en condiciones reductoras de 29.5 KDa. La proteína es fundamentalmente un dímero no unido por enlaces disulfuro, con un contenido de 6.5% de azúcares neutros. Es estable hasta 70-C y en un àmbito de pH de 2 a 10. Aglutina indistintamente los eritrocitos humanos y diferencia entre eritrocitos de origen animal, aglutinando los de conejo y pollo y no los de caballo, cabra, carnero ni rata. La galactosa N-acetil-galactosamina, lactosa y el EDTA inhiben su acción aglutinante, los iones calcio y mangneso son activadores. No se encontró efecto vasopresor al inyectar la lectina itravenosamente en ratas